• Place:
  Berlin
• Course goals:
  In this course you will learn the principles and techniques for the development of robust HPLC Methods. The course will be held in english language.
  You will receive a practical course manuscript and a CD of an HPLC teaching software with the exercizes used in the course and with other typical examples of HPLC method development.
• Target group:
  Pharmacists, chemists and members of R&D or quality control in the pharmaceutical, chemical and food industry.
• The course will be hold by well known experts in the field:
  Dr. Imre Molnar studied chemistry in Saarbrücken with special training in analytical chemistry. He then worked for two years as a post-doctoral fellow with Csaba Horváth at Yale University, USA, on fundamentals of Reversed Phase Chromatography. He has 20 years of experience in the development of HPLC-instruments, column production and HPLC applications and spezializes in pharmaceutical and biopolymer research and analysis. For nearly the same time he is working as a trainer for HPLC. Since 1986 there is a close cooperation with Lloyd R. Snyder, John Dolan and co-workers from LC Resources.Inc. USA, for the joint development of the DryLab-Software.
  Dr.Hans-Jürgen Rieger studied chemistry in Berlin. He work since 7 years for Molnar-Institut as a application chemist and software developer.

First Day

Begin at 9:00 AM Introduction to HPLC-Method Development Reversed Phase Basics - Fundamentals, simple rules Exercise 1. Influence of eluent composition, %B, peaktracking problems Coffee Break Reversed Phase Basics - Secondary equilibria Exercise 2. Speeding up the analysis: Column and flow optimization Characteristics of Columns, stationary phase materials in RPC Lunch Break Critical resolution map and method robustness Exercise 3: Calculation of robustness - Setup and pH example Coffee Break Exercise 4. Temperature optimization Exercise 5: Ternary eluent optimization. End around 5:00 PM - - - - - - - - - - - - - - - - - - - - - - - -

Second day

Begin at 9:00 AM Equivalence and diversity of stationary phases (ColumnMatch) Gradient elution Coffee Break Exercise 6: Gradient method development Method transfer Lunch Break Peak match and data transfer to Drylab Troubleshooting of HPLC methods using computer modeling, precision issues Exercise 7: Automatic gradient development Coffee Break Unexpected results in method development: Neutral compounds and the influence of the organic modifier (Kava-Kava) Exercise 8: Two-dimensional optimiziation tG vs. pH Advanced method development: Anything vs. anything: Two-dimensional simultaneous optimization of any two parameters in HPLC. Discussion End around 5:00 PM

• Participation fee
  The cost of participation for two days is EUR 1.340,- . If you register 6 weeks in advance the cost will be EUR 1.190,- .
  
• Bank tranfer
  Berliner Sparkasse (BLZ 100 500 00), Konto-Nr. 6 5000 7000
  IBAN: DE51 1005 0650 0070 00, BIC/SWIFT-Code: BELADEBE
  Remark: HPLC-Course in Berlin, and your name
  


• Registration
  

  Registration by e-mail:
  info@molnar-institut.com

  or fill out the registration form, and send/fax it back to:

  Molnar-Institut für angewandte Chromatographie
  Schneeglöckchenstr. 47
  D-10407 Berlin
  tel.: +49-30-421-559-0
  fax: +49-30-421-559-99
  
  

Accomodation
For hotels you can have a look at the Berlin Tourist-Info.