According to the Solvophobic Theory (I) a variation of pH, temperature, gradient time or %B or ternary eluent composition makes a continuous variation of the chromatographic selectivity possible. The comprehension and assignment of peaks from a set of systematic experiments is an important first step to control the development process of an HPLC method.
PeakMatch®helps you to bring order into your experiments: Whether it is cutting up double peaks, re-arranging turnover peaks or reducing general complexity, zooming into relevant peak areas and deleting irrelevant bands, PeakMatch®offers you useful functions to prepare your peak table in the best possible way.The simple adjustment of the peak table and the following transfer of the data into DryLab®with one mouseclick are important reasons for the success of PeakMatch®.
During four steps PeakMatch®helps the user to keep survey of his work:
→ Step 1:Data organisation
PeakMatch® structures your experiments and takes care of the documentation, comparison and recognition of the key influences and the method as a whole. The chromatograms and the peak table are displayed completely on one page in the correct order, which facilitates the overview of the complex data, enhancing correct assignments.
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→ Step 2:Peak Tracking
Cell colours imply a good (green), questionable (blue) and false (red) peak tracking. A false assignment of the same substance between different experiments is a common reason for mistakes during method modelling. This is why peak matching is so important: It makes sure that the chromatographer understand, which movements peaks are making, depending on different experimental conditions, and it links the identical substances in a way, that they all end up standing in a single horizontal line. The green colour indicates a well tracked peak table.
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→ Step 3:Data Transfer to DryLab
PeakMatch®transfers all collected data (peak table with retention times, peak areas, eluents, flowrate, column length, column diameter, particle size, gradient range, etc.) into DryLab®with one mouseclick. Within a few seconds DryLab®then simulates a model, consisting of 3 to 4000 pixels, each pixel representing a virtual experiment. In this way we can reduce trial and error to the selection of the few experiments, which are the best ones.
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→Step 4: Validation of the DryLab® model
By comparing the calculated DryLab® model with the original experiments, PeakMatch® validates the best virtual DryLab® models. If simulations and experiments correspond, the match is correct and the DryLab® model can be further evaluated.
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The quality of pharmaceutical products is always approved by HPLC methods.
A safe and robust method is therefore a necessary component of the production process. PeakMatch® and DryLab®help to achieve this aim. They help to structure and organize your work, save time and material for runs and make the laboratory work more predictable, reliable, transferable and successful.
