According to the Solvophobic Theory (I) a variation of pH, temperature, gradient time or %B or ternary eluent composition makes a continuous variation of the chromatographic selectivity possible. The comprehension and assignment of peaks from a set of systematic experiments is an important first step to control the development process of an HPLC method.

PeakMatch^® helps you to bring order into your experiments: Whether it is cutting up double peaks, re-arranging turnover peaks or reducing general complexity, zooming into relevant peak areas and deleting irrelevant bands, PeakMatch^® offers you useful functions to prepare your peak table in the best possible way.The simple adjustment of the peak table and the following transfer of the data into DryLab^® with one mouseclick are important reasons for the success of PeakMatch^®.

During four steps PeakMatch^® helps the user to keep survey of his work:


→ Step 1: Data organisation

PeakMatch^® structures your experiments and takes care of the documentation, comparison and recognition of the key influences and the method as a whole. The chromatograms and the peak table are displayed completely on one page in the correct order, which facilitates the overview of the complex data, enhancing correct assignments.

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→ Step 2: Peak Tracking

Cell colours imply a good (green), questionable (blue) and false (red) peak tracking. A false assignment of the same substance between different experiments is a common reason for mistakes during method modelling. This is why peak matching is so important: It makes sure that the chromatographer understand, which movements peaks are making, depending on different experimental conditions, and it links the identical substances in a way, that they all end up standing in a single horizontal line. The green colour indicates a well tracked peak table.

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→ Step 3: Data Transfer to DryLab

PeakMatch^® transfers all collected data (peak table with retention times, peak areas, eluents, flowrate, column length, column diameter, particle size, gradient range, etc.) into DryLab^® with one mouseclick. Within a few seconds DryLab^® then simulates a model, consisting of 3 to 4000 pixels, each pixel representing a virtual experiment. In this way we can reduce trial and error to the selection of the few experiments, which are the best ones.

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→Step 4: Validation of the DryLab^® model

By comparing the calculated DryLab^® model with the original experiments, PeakMatch^® validates the best virtual DryLab^® models. If simulations and experiments correspond, the match is correct and the DryLab^® model can be further evaluated.

              
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The quality of pharmaceutical products is always approved by HPLC methods. A safe and robust method is therefore a necessary component of the production process. PeakMatch^® and DryLab^® help to achieve this aim. They help to structure and organize your work, save time and material for runs and make the laboratory work more predictable, reliable, transferable and successful.